The antiserum was produced against synthesized peptide derived from human Histone H2A around the phosphorylation site of Thr121.
Immunogen Range
81-130
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:1000
Specificity
Histone H2A (Phospho-Thr121) Antibody detects endogenous levels of Histone H2A only when phosphorylated at Thr121.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Histone H2A (Phospho-Thr121) Antibody. The picture on the right is blocked with the phospho peptide.