The antiserum was produced against synthesized peptide derived from human MKK6 around the phosphorylation site of Ser207.
Immunogen Range
173-222
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
MKK6 (Phospho-Ser207) Antibody detects endogenous levels of MKK6 only when phosphorylated at Ser207.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAP2K6
Database Links
Gene ID: 5608 Swiss-Prot #: P52564
OMIM #: 601254 Unigene #: Hs.463978
Target Modification
Phospho
Modification Sites
H:S207 M:S207 R:S207
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MKK6 (Phospho-Ser207) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from MDA-MB-435 cells, using MKK6 (Phospho-Ser207) Antibody. The lane on the left is blocked with the phospho peptide.