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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | IRS-1 (Phospho-Ser636) Antibody |
| Synonyms | Insulin receptor substrate-1; IRS-1; IRS1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser636. |
| Immunogen Range | 603-652 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 |
| Specificity | IRS-1 (Phospho-Ser636) Antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser636. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | IRS1 |
| Database Links |
Gene ID: 3667 Swiss-Prot #: P35568 OMIM #: 125853/147545 Unigene #: Hs.471508 |
| Target Modification | Phospho |
| Modification Sites | H:S636 M:S632 R:S632 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of MCF7 cells, using IRS-1 (Phospho-Ser636) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser636) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 3 |
 Western blot analysis of lysates from 293 cells and COLO205 cells, using IRS-1 (Phospho-Ser636) Antibody. The lane on the right is blocked with the phospho peptide. |
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