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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
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| Product Name | AFX (Phospho-Ser197) Antibody |
| Synonyms | AFX; AFX1; Afxh; forkhead box protein O4; forkhead domain AFX1; MLLT7; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 (AFX1) |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AFX around the phosphorylation site of Ser197. |
| Immunogen Range | 164-213 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 |
| Specificity | AFX (Phospho-Ser197) Antibody detects endogenous levels of AFX only when phosphorylated at Ser197. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | FOXO4 |
| Database Links |
Gene ID: 4303 Swiss-Prot #: P98177 OMIM #: 300033 Unigene #: Hs.584654 |
| Target Modification | Phospho |
| Modification Sites | H:S197 M:S197 |
| Application Images | |
| Image 1 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AFX (Phospho-Ser197) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Western blot analysis of lysates from 293 cells treated with serum, using AFX (Phospho-Ser197) Antibody. The lane on the right is blocked with the phospho peptide. |
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