Gibco/SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red/500 mL/A2494301

Description

SILACAdvancedDMEM/F-12FlexisAdvancedDMEM/F-12withoutL-arginine,L-glutamine,andL-lysineforuseduringSILACproteinlabelingwithstableisotopiclabeledlysineand/orarginine.AdvancedDMEM/F-12isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells,whichreducestheFBSrequirementsby50-90%withoutanychangesingrowthrateormorphology.Whensupplementedwith1-2%FBS,AdvancedDMEM/F-12Flexiscapableofsupportingcellularproliferationandmaximumcelldensitiescomparabletotheconventionalbasalformulationsupplementedwith5-10%FBS.CellssuccessfullyculturedinAdvancedDMEM/F-12withnoadaptationincludeJurkat,WI-38,SP2,Vero,andMRC-5.WeofferavarietyofDMEM/F-12modificationsforarangeofapplications.Findtherightformulationusingthemediaselectortool.
AdvancedDMEM/F-12isuniquefromothermediaduetoadditionofthefollowingingredientstoallowforserumreduction:ethanolamine,glutathione,ascorbicacid,insulin,transferrin,AlbuMAX®Ilipid-richbovineserumalbuminforcellculture,andthetraceelementssodiumselenite,ammoniummetavanadate,cupricsulfate,andmanganouschloride.AdvancedDMEM/F-12typicallyrequiressupplementationwith1-5%FetalBovineSerumand4mML-glutamineorGlutaMAX™supplement(optional).SILACproteinlabelingexperimentsshouldbeconductedusingGibco™DialyzedFBS.TheFBSconcentrationmustbeoptimizedforeachcelllinetoobtainmaximumserumreduction.AdvancedDMEM/F-12usesasodiumbicarbonatebuffersystem(3.7g/L),andthereforerequiresa5-10%CO₂environmenttomaintainphysiologicalpH.

cGMPManufacturingandQualitySystem
SILACAdvancedDMEM/F-12FlexismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.