Description
SILACDMEMFlexisDulbecco’sModifiedEagleMedium(DMEM)withoutL-glutamine,L-lysine,andL-arginineforuseduringSILACproteinlabelingwithstableisotopiclabeledlysineand/orarginine.DMEMisawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEMincludeprimaryfibroblasts,neurons,glialcells,HUVECs,andsmoothmusclecells,aswellascelllinessuchasHeLa,293,Cos-7,andPC-12.Weofferavarietyof
Gibco™DMEMmodificationsforarangeofapplications.Findtherightmodificationusingthemediaselectortool.
ThisDMEMismodifiedasfollows:With | Without |
| •Glucose |
| •Phenolred |
| •L-arginine |
| •L-glutamine |
| •L-lysine |
| •Sodiumpyruvate |
DMEMisuniquefromothermediaasitcontains4timestheconcentrationsofaminoacidsandvitaminsthantheoriginalEagle"sMinimalEssentialMedium.DMEMwasoriginallyformulatedwithlowglucose(1g/L)andsodiumpyruvate,butisoftenusedwithhigherglucoselevels,withorwithoutsodiumpyruvate.SILACDMEMFlexhasbeenfurthermodifiedtonotcontainlysine,arginine,andglutamine.DMEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,DMEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).SILACproteinlabelingexperimentsshouldbeconductedusingGibco™DialyzedFBS.DMEMusesasodiumbicarbonatebuffersystem(3.7g/L),sorequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
cGMPManufacturingandQualitySystemSILACDMEMFlexismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
ForResearchUseorFurtherManufacturing.Notforuseindiagnosticprocedures.