Description
TheExpiFectamine™293TransfectionKitisacorecomponentoftheExpi293™ExpressionSystem.ItisdesignedfortransienttransfectionofhighdensityculturesofHEK(humanembryonickidney)293cells.Thehigh-efficiency,cationic,lipid-basedtransfectionreagentandtransfectionenhancersaredesignedtopowerthehighestposs
IBLelevelofproteinexpressionfromExpi293F™cellsculturedinExpi293™ExpressionMedium
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Designedspecificallyfortransfectionofhighdensitys
USPensioncellculture,withmatchingtransfectionenhancersthatboosttransfectionperformanceandproteinexpression
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Achievesproteinyields2-to10-foldhigherthanothertransfectionreagentsusedonhighdensity293cellcultures
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Employsthesametransientexpressionprotocolstypicallyusedincurrentlow-density293suspensionculturesystemstoeasilyswitchfromlowdensitysystemstothehighyield,highdensityExpi293™ExpressionSystem
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Providesrobustandreproducibletransfectionresults,givingusersgreaterconfidenceintheirresults
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Scaletransfectionsforculturevolumesoflessthan1mLtogreaterthan10liters,whilemaintainingequivalentvolumetricproteinyields
TheExpiFectamine™293TransfectionKitisspecificallydesignedtoworkwithExpi293F™cellsintheExpi293™ExpressionSystem.Thetransfectionkitconsistsofthecationiclipid-basedExpiFectamine™293ReagentandExpiFectamine™293TransfectionEnhancers1and2.Theenhancersareadded16to18hourspost-transfectiontoboosttransfection,cellvi
ABIlity,andproteinexpression.TheprotocolforuseofExpiFectamine™293Transfectioncomponentsisverysimilartotransfectionprotocolsforothertransientexpressionreagents,suchas293fectinandFreeStyle™MAX.
ForResearchUseOnly.Notforuseindiagnosticprocedures.