Description
TheExpiFectamine™293TransfectionKitisacorecomponentoftheExpi293™ExpressionSystem.ItisdesignedfortransienttransfectionofhighdensityculturesofHEK(humanembryonickidney)293cells.Thehigh-efficiency,cationic,lipid-basedtransfectionreagentandtransfectionenhancersaredesignedtopowerthehighestposs
IBLelevelofproteinexpressionfromExpi293F™cellsculturedinExpi293™ExpressionMedium•
Designedspecificallyfortransfectionofhighdensitys
USPensioncellculture,withmatchingtransfectionenhancersthatboosttransfectionperformanceandproteinexpression•
Achievesproteinyields2-to10-foldhigherthanothertransfectionreagentsusedonhighdensity293cellcultures•
Employsthesametransientexpressionprotocolstypicallyusedincurrentlow-density293suspensionculturesystemstoeasilyswitchfromlowdensitysystemstothehighyield,highdensityExpi293™ExpressionSystem•
Providesrobustandreproducibletransfectionresults,givingusersgreaterconfidenceintheirresults•
Scaletransfectionsforculturevolumesoflessthan1mLtogreaterthan10liters,whilemaintainingequivalentvolumetricproteinyieldsTheExpiFectamine™293TransfectionKitisspecificallydesignedtoworkwithExpi293F™cellsintheExpi293™ExpressionSystem.Thetransfectionkitconsistsofthecationiclipid-basedExpiFectamine™293ReagentandExpiFectamine™293TransfectionEnhancers1and2.Theenhancersareadded16to18hourspost-transfectiontoboosttransfection,cellvi
ABIlity,andproteinexpression.TheprotocolforuseofExpiFectamine™293Transfectioncomponentsisverysimilartotransfectionprotocolsforothertransientexpressionreagents,suchas293fectinandFreeStyle™MAX.
ForResearchUseOnly.Notforuseindiagnosticprocedures.