Description
MinimumEssentialMedium(MEM)isoneofthemostcommonlyusedofallcellculturemedia.MEMcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingHeLa,BHK-21,293,HEP-2,HT-1080,MCF-7,fibroblasts,andprimaryratastrocytes.Weofferavarietyof
Gibco®MEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingourmediaselectortool.
ThisMEMismodifiedasfollows:
With | Without |
•Phenolred | •L-glutamine |
•6g/LD-glucose | •HEPES |
Gibco®MEM,developedbyHarryEagle,wasbasedonhisearlierformulationofBasalMediumEagle(BME).ManyothermodificationsofMEMfollowed,includingGlasgow"sMEM,MEMα,DMEM,andTemin"sModification.MEMisavailablewithEarle"ssaltsforuseinaCO
2incubator,orwithHanks"saltsforusewithoutCO
2.
ProductIntendedUseForResearchUseOnly.Notintendedforanimalorhumandiagnosticortherapeuticuse.
cGMPManufacturingandQualitySystemGibco®MEMismanufacturedatacGMPcompliantfacility,locatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
MEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMrequiressupplementation,commonlywith10%fetalbovineserum(FBS).MEMusesasodiumbicarbonatebuffersystem(2.2g/L)andthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.