Description
RoswellParkMemorialInstitute(RPMI)1640mediumwasoriginallydevelopedtoculturehumanleukemiccellsins
USPensionandasamonolayer.RPMI1640mediumhassincebeenfoundsuitableforavarietyofmammaliancellsincludingHeLa,Jurkat,MCF-7,PC12,PBMC,astrocytes,andcarcinomas.Weofferavarietyof
Gibco®RPMI1640modificationsforarangeofcellcultureapplications.Findtherightformulationusingourmediaselectortool.
ThisRPMImediumismodifiedasfollows:
With | Without |
•L-glutamine | •Methionine |
Gibco®RPMI1640mediumisuniquefromothermediabecauseitcontainsthereducingagentglutathioneandhighconcentrationsofvitamins.RPMI1640containsbiotin,vitaminB
12,andPABA,whicharenotfoundinEagle"sMinimalEssentialMediumorDulbecco"sModifiedEagleMedium.Inaddition,thevitaminsinositolandcholinearepresentinveryhighconcentrations.
ProductIntendedUseForResearchUseOnly.Notintendedforanimalorhumandiagnosticortherapeuticuse.
cGMPManufacturingandQualitySystemThisGibco®RPMImediumismanufacturedatacGMPcompliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
RPMI1640mediumcontainsnoproteins,lipids,orgrowthfactors.Therefore,RPMI1640mediumrequiressupplementation,commonlywith10%fetalbovineserum(FBS).RPMI1640mediumusesasodiumbicarbonatebuffersystem(2.0g/L)andthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.