Description
Lipofectamine®LTXReagentisaplasmidtransfectionreagentthatofferstherightbalanceofpotencyandgentlenessforyourcells,resultinginthehighestposs
IBLetransfectionefficienciesandvi
ABIlities.Lipofectamine®LTXReagentisanadvancedsolutionforgeneexpressioninbothcommonandchallengingcelltypes.NootherplasmidDNA-specifictransfectionreagentcanmatchtheefficiency,convenience,andgentlenessofLipofectamine®LTXReagentfortransfectionofprimary,challenging,andsensitivecelllines.Lipofectamine®LTXReagentissynthesizedfrom100%animalorigin-freecomponents,makingiteasytovalidatetheabsenceofzoonoticdiseases,suchasBSEorviruses,inresearchexperimentsorcelllines.
Lipofectamine®LTXReagentoffers:
•Superiordelivery—transfectabroadrangeofcelltypes,includingprimary,challenging,andsensitivecells,withhighefficiency
•Superiorperformance—combinehightransfectionefficiencywithmaximal(>90%)cellviability
•Optimizedprotocols—rapid,simpleprotocolsprovidedformostcelllines
•Ahighlyeffectivereagenttogeneratestablecelllines
TransfectprimarycellsefficientlyPrimarycellsareoftendifficulttotransfect.Lipofectamine®LTXReagent,whencombinedwithPLUS™Reagent,providesthehighestleveloftransfectioninprimarycellsofanylipid-orpolymer-basedreagent.ManyprimarycelltypeshavebeensuccessfullytransfectedwithLipofectamine®LTXReagent,includingprimaryneural
Progenitorcells.
SuperiordeliveryintochallengingcellsWhenitcomestoachievinghighlevelsofproteinexpressioninRAW267.3macrophages,Lipofectamine®LTXReagentoutperformscompetingtransfectionreagents(seefigure).Achievinghigh-leveltransgeneexpressionincellsthataretr
ADItionallyrefractorytotransfectionallowsyoutoobtainresultsthatwerepreviouslyunattainable.
BroadcompatibilitywithcelllinesInadditiontobeingexcellentfortransfectingprimaryandchallengingcells,Lipofectamine®LTXReagentprovidesyouwiththemostversatileapproachtotransfectingestablishedcellslines,includingthosethataresensitivetoothertransfectionreagents.UseourTransfectionSelectionTooltohelpyouselectthebesttransfectionconditionsforthecellswithwhichyouwork.
StabletransfectionLipofectamine®LTXstrikestherightbalancebetweenpotencyandgentleness,givingyouthehighestpossiblepercentageofsuccessfullytransfectedcells.ThismakesLipofectamine®LTXReagentthequickestandeasiestreagentforestablishingstabletransfectantsfromchallengingorrefractorycelltypes.
Simple,rapid,serum-compatibleprotocolLipofectamine®LTXReagentoffersastreamlinedprotocol—noneedtoremovetransfectioncomplexesorchange/addmediumfollowingtransfection.Asimpleoptimizationprotocolhelpsyougetthebestresultsquickly,soyou"llspendlesshands-ontime,andgetfasterresults.
ForResearchUseOnly.Notforuseindiagnosticprocedures.