Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | VAV2(Phospho-Tyr142)Antibody |
Synonyms | Vav-2protein;GuaninenucleotideexchangefactorVAV2;VAV-2 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanVAV2aroundthephosphorylationsiteofTyr142. |
ImmunogenRange | 108-157 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:1000 |
Specificity | VAV2(Phospho-Tyr142)AntibodydetectsendogenouslevelsofVAV2onlywhenphosphorylatedatTyr142. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | VAV2 |
DatabaseLinks | GeneID: 7410 Swiss-Prot#: P52735 OMIM#: 600428 Unigene#: Hs.369921/Hs.689325 |
TargetModification | Phospho |
ModificationSites | H:Y142M:Y142 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofNIH/3T3cells,usingVAV2(Phospho-Tyr142)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanbrain,usingVAV2(Phospho-Tyr142)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromHeLacellsandK562cellstreatedwithTNF20ng/ml30",usingVAV2(Phospho-Tyr142)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |