The antiserum was produced against synthesized peptide derived from human SEK1/MKK4/JNKK1 around the phosphorylation site of Ser257.
Immunogen Range
223-272
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:40000
Specificity
SEK1/MKK4/JNKK1 (Phospho-Ser257) Antibody detects endogenous levels of SEK1/MKK4/JNKK1 only when phosphorylated at Ser257.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAP2K4
Database Links
Gene ID: 6416 Swiss-Prot #: P45985
OMIM #: 601335 Unigene #: Hs.514681
Target Modification
Phospho
Modification Sites
H:S257 M:S255 R:S135
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using SEK1/MKK4/JNKK1 (Phospho-Ser257) Antibody. The picture on the right is blocked with the phospho peptide.