The antiserum was produced against synthesized peptide derived from human IL-10R alpha around the phosphorylation site of Tyr496.
Immunogen Range
462-511
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
IL-10R alpha (Phospho-Tyr496) Antibody detects endogenous levels of IL-10R alpha only when phosphorylated at Tyr496.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
IL10RA
Database Links
Gene ID: 3587 Swiss-Prot #: Q13651
OMIM #: 146933 Unigene #: Hs.504035
Target Modification
Phospho
Modification Sites
H:Y496
Application Images
Image 1
Western blot analysis of lysates from HUVEC cells, using IL-10R alpha (Phospho-Tyr496) Antibody. The lane on the right is blocked with the phospho peptide.