Description
RPMI1640Mediumwasoriginallydevelopedtoculturehumanleukemiccellsins
USPensionandasamonolayer.RoswellParkMemorialInstitute(RPMI)1640Mediumhassincebeenfoundsuitableforavarietyofmammaliancells,includingHeLa,Jurkat,MCF-7,PC12,PBMC,astrocytes,andcarcinomas.WeofferavarietyofRPMI1640Mediummodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisRPMIismodifiedasfollows:
With | |
L-glutamine | |
HEPES | |
PhenolRed | |
SodiumPyruvate | |
Highglucose | |
Thecompleteformulationisavailable.
RPMI1640Mediumisuniquefromothermediabecauseitcontainsthereducingagentglutathioneandhighconcentrationsofvitamins.RPMI1640Mediumcontainsbiotin,vitaminB
12,andPABA,whicharenotfoundinEagle"sMinimalEssentialMediumorDulbecco"sModifiedEagleMedium.Inaddition,thevitaminsinositolandcholinearepresentinveryhighconcentrations.ThisRPMIformulationhasbeenmodifiedby
ATCCtocontainhighglucose,lowsodiumbicarbonate,sodiumpyruvate,HEPES,andlowL-glutamine.RPMI1640Mediumcontainsnoproteins,lipids,orgrowthfactors.Therefore,RPMI1640Mediumrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).RPMI1640Mediumusesasodiumbicarbonatebuffersystem(1.5g/L)andthereforerequiresa5–7%CO
2environmenttomaintainphysiologicalpH.
cGMPmanufacturingandqualitysystemRPMI1640MediumismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
ForResearchUseOnly.Notforuseindiagnosticprocedures.