Description
MEMα(MinimumEssentialMediumα)iswidelyusedformammaliancellcultureaswellasselectionfortransfectedDHFR-negativecells.MEMαcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingkeratinocytes,primaryratastrocytes,andhumanmelanomacells.WeofferavarietyofMEMαmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisMEMαismodifiedasfollows:With | Without |
Deoxyribonucleosides | Ascorbicacid |
Ribonucleosides | |
L-glutamine | |
Thecompleteformulationisavailable.
UsingMEMαMEMαisamodificationofMinimumEssentialMedium(MEM)thatcontainsnon-essentialaminoacids,sodiumpyruvate,lipoicacid,vitaminB
12,biotin,andascorbicacid.MEMαisavailablewithoutnucleosidesforuseasaselectionmediumforDG44andotherDHFR-negativecells.ThisMEMismodifiedwithoutascorbicacid.MEMαcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMαrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).MEMαusesasodiumbicarbonatebuffersystem(2.2g/L),andthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.
cGMPmanufacturingandqualitysystemMEMαismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
ForResearchUseOnly.Notforuseindiagnosticprocedures.