Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | CRYAB(Phospho-Ser45)Antibody |
Synonyms | AlphacrystallinBchain;Alpha(B)-crystallin;CRYA2;P23;Rosenthalfibercomponent;crystallin;alphaB |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCRYABaroundthephosphorylationsiteofSer45. |
ImmunogenRange | 21-70 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:10000 |
Specificity | CRYAB(Phospho-Ser45)AntibodydetectsendogenouslevelsofCRYABonlywhenphosphorylatedatSer45. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | CRYAB |
DatabaseLinks | GeneID: 1410 Swiss-Prot#: P02511 OMIM#: 123590/608810 Unigene#: Hs.408767 |
TargetModification | Phospho |
ModificationSites | H:S45M:S45R:S45 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanheart,usingCRYAB(Phospho-Ser45)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromCOS7cellstreatedwithanisomycin25ug/ml30",usingCRYAB(Phospho-Ser45)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |