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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | Retinoblastoma (Phospho-Thr821) Antibody |
| Synonyms | P105-RB; PP105; PP110; RB-1; RB1; retinoblastoma gene; Retinoblastoma-associated protein |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Thr821. |
| Immunogen Range | 791-840 |
| Clonality | Polyclonal |
| Application Dilutions | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 |
| Specificity | Retinoblastoma (Phospho-Thr821) Antibody detects endogenous levels of Retinoblastoma only when phosphorylated at Thr821. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | RB1 |
| Database Links |
Gene ID: 5925 Swiss-Prot #: P06400 OMIM #: 109800/180200/259500 Unigene #: Hs.408528 |
| Target Modification | Phospho |
| Modification Sites | H:T821 M:T814 R:T792 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of HeLa cells, using Retinoblastoma (Phospho-Thr821) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Immunohistochemistry analysis of paraffin-embedded human testis, using Retinoblastoma (Phospho-Thr821) Antibody. The picture on the right is blocked with the phospho peptide. |
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