Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | Ataxin1(Phospho-Ser776)Antibody |
Synonyms | ATX1;ATXN1;SCA1;Spinocerebellarataxiatype1protein;Spinocerebellarataxiatype1proteinhomolog |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanAtaxin1aroundthephosphorylationsiteofSer776. |
ImmunogenRange | 742-791 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IF:1:100~1:500ELISA:1:10000 |
Specificity | Ataxin1(Phospho-Ser776)AntibodydetectsendogenouslevelsofAtaxin1onlywhenphosphorylatedatSer776. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | ATXN1 |
DatabaseLinks | GeneID: 6310 Swiss-Prot#: P54253 OMIM#: 164400/601556 Unigene#: Hs.434961 |
TargetModification | Phospho |
ModificationSites | H:S776M:S752 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofNIH/3T3cells,usingAtaxin1(Phospho-Ser776)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromHepG2cellstreatedwithAdriamycin0.5uM5h,usingAtaxin1(Phospho-Ser776)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |