Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | Keratin18(Phospho-Ser33)Antibody |
Synonyms | CK18;CK18;CYK18;CytokeratinendoB;K18;K1C18;KERD;KRT1-18;KRT18;Keratin;KeratinD;Keratin;typeIcytoskeletal18;cytokeratin18;typeIcytoskeletal18 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanKeratin18aroundthephosphorylationsiteofSer33. |
ImmunogenRange | Jan-50 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:5000 |
Specificity | Keratin18(Phospho-Ser33)AntibodydetectsendogenouslevelsofKeratin18onlywhenphosphorylatedatSer33. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | KRT18 |
DatabaseLinks | GeneID: 3875 Swiss-Prot#: P05783 OMIM#: 148070/215600 Unigene#: Hs.406013 |
TargetModification | Phospho |
ModificationSites | H:S34M:S35R:S35 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHeLacells,usingKeratin18(Phospho-Ser33)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingKeratin18(Phospho-Ser33)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromHT29cells,usingKeratin18(Phospho-Ser33)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |