CAS Number: 355137-38-1Molecular Weight: 706.23Salt Form: TFAPurity: >96%Sequence (3-letter): Ac-Asp-Met-Gln-Asp-AMCSequence (1-letter): Ac-DMQD-AMCStorage: -20 °C or below
Ac-Asp-Met-Gln-Asp-AMC is a fluorogenic substrate for Caspase 3 (Apopain). Caspase 3 is a cysteine protease that is rapidly activated during apoptosis and that cleaves several substrates, including poly(ADP-ribose) polymerase (PARP). Caspase 3 is activated by Caspases 8, 9 and 10 and cleaves and activates caspase 6 and 7. Caspase 3 cleaves the amyloid precursor protein (APP) contributing to Amyloid beta formation and has been implicated in Alzheimer’s disease and proposed as a target for drug discovery. Ac-DMQD-AMC is reported to be a more specific caspase-3 substrate than Ac-DEVD-AMC.
Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.
References
1. Talanian, R.V. et al. (1997) “Substrate Specificities of Caspase Family Proteases” J. Biol. Chem. 272 (15): 9677–9682.2.Nagane, M. et al. (1998) “Drug resistance of human glioblastoma cells conferred by a tumor-specific mutant epidermal growth factor receptor through modulation of Bcl-XL and caspase-3-like proteases” Proc. Natl. Acad. Sci. USA 95 (10): 5724–5729.3) Troy T. Rohn, Elizabeth Head “Caspases as Therapeutic Targets in Alzheimer’s Disease: Is It Time to “Cut” to the Chase?” Int J Clin Exp Pathol. 2009; 2(2): 108–118.
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