Properties:
RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHPLCColumns,TOYOPEARLBulkMedia&Accessories,ToyoScreenHydrophobicInteractionProcessDevelopmentColumns, |
material | aluminumseal |
| polypropyleneandpolyethylenefrit |
packaging | pkgof6×5 mL |
mfr.no. | Tosoh,21373 |
parameter | ≤50%organicsolvent |
| 1-5mL/minflowrate |
| 20mL/minmax.flowrate |
| 3barmax.pressure |
L×I.D. | 3 cm×14.6 mm |
matrix | hydroxylatedmethacrylicpolymer |
matrixactivegroup | etherphase |
particlesize | 65 μm |
operatingpH | 1-13 |
capacity | 20 g/L±10adsorptioncapacity(lysozyme) |
modeofchromatography | Hydrophobicinteraction(HIC) |
Description:
Generaldescription
Everylargescalecommercialpurificationbeginsatasmallscaleinamethoddevelopmentlabbyevaluatingretentionandselectivityofthetargetproteinversusimpurities,hostcellproteins,DNA,etc.,onmultipleresins,oftenfromdifferentvendors.Dependingontheconstraints,theprocessdevelopmentengineercaneitherpurchasebulkresinstopackseveralsmallcolumnswithdifferentresinsortakeadvantageofthespeedandconvenienceofpre-packedcolumnsforresinevaluation.Pre-packedToyoScreen®ProcessDevelopmentColumnsareavailablein1mLand5mLvolumespackedwiththemostpopularToyopearl®resins.ToyoScreencolumnscanbeusedtoquicklyestablishoptimumelutionconditions.VaryingpH,salttype,grADIentconditionsandflowratearecommonexperimentalparametersexplored.InitialresultsfromresinscreeningandoptimizationwithToyoScreencolumnsaccuratelypredicttheseparationbehavioratlargerscale.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
Specifications
Cleaninplacewith0.5MNaOHor0.1MHCl.
LegalInformation
ToyoScreenisaregisteredtrademarkofTosohCorporation
ToyopearlisaregisteredtrademarkofTosohCorporation