Synonym:TSKgel®Chelate-5PWHPLCColumnandGuardgelKitProperties:
RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelAffinityHPLCColumns,TSKgelHPLCColumns,TOYOPEARLBulkMedia&Accessories |
Description:
Generaldescription
Highefficiency,resin-basedTSKgelaffinitycolumns(10μmparticles)separateorpurifymanyenzymesandotherproteins.
TSKgelChelate-5PWcolumnsutilizethetendencyofiminodiaceticacid(IDA)toformacomplexwithmetalions,suchasZn2+,Ni2+andCu2+.Thecolumnisloadedwithdivalentmetalionsbychelation.PeptidesandproteinscontaininghistidineresidueswillnormallyadsorbtothesechelatedionsatneutralpH.Theretainedcompoundscanbeelutedwithbuffercontainingimidazoleorglycine.Thekeytomakingsuccessfuluseofthisretentionmechanismistheproperselectionofmetalionsforchelationandtheelutionbuffertodesorbtheanalytes.Ingeneral,Cu2+,interactsbetterwithproteins;however,resolutionisusuallyenhancedwithZn2+.AgrADIentmobilephasecontainingincreasingimidazoleorglycineconcentrationsisusedtoelutetheretainedcompounds.Alternatively,adecreasingpHgradientcanbeusedforelutionpurposesaswell.
Applicationsinclude:immunoglobulins,transferrin,lectins,milkproteins,membraneproteinsandpeptides.
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LegalInformation
TSKgelisaregisteredtrademarkofTosohCorporation