Immunogen:
Fraction of bovine brain GABA-A receptor affinity purified against immobilized
benzodiazepine Ro7-1986/1 (1988 de Blas et al J Neurosci PMID 2828565, 1988 Vitorica
et al J Neurosci PMID 2828566). Target initially identified as a GABA-A receptor by:
- rat brain immunocytochemistry staining pattern via light microscopy identical to that seen
via ligand autoradiography with 3H-muscimol
- immunoprecipitation of 3H-muscimol-binding activity of the purified receptor complex
- recognition of ~57 kDa protein in immunoblots against crude rat brain membrane fractions
Binding epitope identified to be within amino acids 26-40 (QSVNDPGNMSFVKET,
extracellular N-terminus, 1992 Ewert et al Brain Res, PMID 1377081) of human
GABA-A-R-Beta3 (also known as Gamma-aminobutyric acid receptor subunit beta-
3, accession number P28472)
Mouse: 100% identity (15/15 amino acids identical)
Rat: 100% identity (15/15 amino acids identical)
86% identity (13/15 amino acids identical, QSVNDPSNMSLVKET) with GABA-A-R-Beta2
66% identity (10/15 amino acids identical, HSTNEPSNMSYVKET) with GABA-A-R-Beta1
Hybridoma kindly donated by Angel de Blas, University of Connecticut. This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.
Specifications:
Target
GABA-A-R-Beta2/3
Applications
Immunoblot (IB) Immunocytochemistry (ICC) Immuno-gold EM Immunohistochemistry (IHC) Immunoprecipitation (IP)
Clone
62-3G1
IgG Isotype
IgG1
Species Reactivity
Bovine (B) Chicken (Chk) Human (H) Mouse (M) Rat (R)
Validation
Br-IB Br-IHC T
Type
Purified
Format
100 ul
Cross Reactivity
Cross-reacts with GABA-A-R-Beta2 Does not cross-react with GABA-A-RBeta1