Thisantibodyshowstheexpectedimmunoperoxidase-diaminobenzidine/immunofluorescencestainingpatternwhenusedtostainbrainsections.
TCSupernatantHybridomatissue-culturesupernatant(5mL)generallycontaining10-50ug/mLmonoclonalantibody(clone-dependent)canbeaveryeconomicalchoice.SupernatantissuitableformostapplicationsincludingWesternBlot,immunocytochemistry,immunohistochemistryetc.Forapplicationsrequiringhigherantibodyconcentrationorpurity(suchasimmunoprecipitation,microinjection,orconjugation)itisrecommendedthatyoupurchasethepurifiedformoftheantibody(suppliedat1mg/mL).
PurifiedmAbsProducedbyinvitrobioreactorcultureofhybridomalinesfollowedbyProteinAaffinitychromatography.PurifiedmAbsare>90%specificantibodyat1mg/mL.