Gibco/RPMI 1640 Medium, GlutaMAX Supplement, HEPES/500 mL/72400047

Description

RPMI1640Medium(RoswellParkMemorialInstitute1640medium)wasoriginallydevelopedtoculturehumanleukemiccellsinsUSPensionandasamonolayer.RPMI1640hassincebeenfoundsuitableforavarietyofmammaliancells,includingHeLa,Jurkat,MCF-7,PC12,PBMC,astrocytes,andcarcinomas.WeofferavarietyofRPMI1640modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
Thecompleteformulationisavailable.

UsingRPMI1640Medium
RPMI1640mediumisuniquefromothermediabecauseitcontainsthereducingagentglutathioneandhighconcentrationsofvitamins.RPMI1640mediumcontainsbiotin,vitaminB₁₂andPABA,whicharenotfoundinEagle"sMinimalEssentialMediumorDulbecco"sModifiedEagleMedium.Inaddition,thevitaminsinositolandcholinearepresentinveryhighconcentrations.RPMI1640mediumwithGlutaMAX™supplementminimizestoxicammoniabuild-upandimprovescellviABIlityandgrowthinaneasy-to-useformat.RPMI1640mediumcontainsnoproteins,lipids,orgrowthfactors.Therefore,RPMI1640mediumrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).RPMI1640mediumusesasodiumbicarbonatebuffersystem(2.0g/L),andthereforerequiresa5–10%CO₂environmenttomaintainphysiologicalpH.

Productintendeduse
Forinvitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.CustomersusingGibco®RPMI1640inamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromustoreferenceourTypeIIDrugMasterFile(DMF).

cGMPmanufacturingandqualitysystem
RPMI1640ismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalRPMI1640productmadeinourScotlandfacility(72400-054).ThisfacilityisalsoregisteredwiththeFDAasamedicaldevicemanufacturerandcertifiedtotheISO13485standard.