Capillary Dimensions,
HPLC,
HPLC & FPLC Columns for Biopolymer Separations
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material
stainless steel hardware (column)
description
Shell thickness (0.6 μm)
Solid Core (3.5 μm)
feature
endcapped
extent of labeling
1.5% carbon loading
parameter
600 bar max. pressure
90 °C max. temp.
application(s)
HPLC: suitable
L × I.D.
5 cm × 1 mm
surface coverage
2.2 μmol/m2
matrix
spherical silica particle platform
matrix active group
cyano phase
particle size
5 μm
pore size
160 Å
surface area
60 m2/g
operating pH range
1 - 8
suitability
suitable for L10 per USP
mode of chromatography
Reversed phase
Description:
General description
BIOshell™ 160 Å Peptide CN columns provide an alternative selectivity to the traditional C18 bonded phases. The introduction of the cyano group enables peptides and small proteins to be separated due to a combination of hydrophobicity and dipole-dipole interactions making this column an excellent choice for biomolecules that are not resolved on traditional C18 columns. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected cyanopropyl ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C).
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