AminereactiveCyanine7,nearinfraredfluorescentdye,animprovedanalogofCy7®.
NIRfluorophorescanbeusedtotakeadvantageofnearinfraredwindowofBIOLOGicaltissues-increasedtransparencyoftissuesinthisspectralregionallowstocarryoutinvivoimaging.
ThisreagentcanbeutilizedtoproduceCyanine7-labeledbiomoleculesforsubsequentuseinvariousinvivoresearch,anddrugdesignrelatedexperiments.
ThestructureofCyanine7featuresrigidizeddesignofcentralpolymethynechain.Thismolecularreinforcementallowstoincreasequantumyieldby20%comparedwithparentstructure,increasingfluorescencebrightness.
Thisreagentrequiresorganicco-solventforthelabeling(pleaseseeRecommendedProtocolssectionbelow).Water-solubleCyanine7NHSesterisalsoavailable,andrecommendedforproteinNIRlabeling.
Generalproperties
Appearance: | greenpowder |
Molecularweight: | 682.29 |
Molecularformula: | C41H48ClN3O4 |
Solubility: | solubleinorganicsolvents(DMSO,DMF,dichloromethane),lowsolubilityinwater |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
Excitationmaximum,nm: | 750 |
ε,L⋅mol−1⋅cm−1: | 199000 |
Emissionmaximum,nm: | 773 |
Fluorescencequantumyield: | 0.3 |
CF260: | 0.022 |
CF280: | 0.029 |