ROX(rhodamineXakaR101)isahighlyfluorescent,andphotostablerhodaminedyeforvariousapplications.ROXlabeledoligonucleotideprobesareoftenusedinqPCR,andqPCRinstrumentshaveROXchannel.
Thisisreactivedyeforthelabelingofamino-groupsinpeptides,proteins,andamino-oligonucleotides.Puresingleisomer.
ThisdyecanreplaceAlexaFluor568,TexasRed.
Generalproperties
Appearance: | darkcrimsonpowder |
MassspecM+increment: | 516.2 |
Molecularweight: | 631.67 |
CASnumber: | 117491-83-5 |
Molecularformula: | C37H33N3O7 |
Solubility: | goodinpolarorganicsolvents(DMF,DMSO),lowinwater |
Qualitycontrol: | NMR1H,HPLC-MS(80+%,balancemostlyfreecarboxylicacid) |
Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
Excitationmaximum,nm: | 570 |
ε,L⋅mol−1⋅cm−1: | 82000 |
Emissionmaximum,nm: | 591 |
Fluorescencequantumyield: | 1.0 |