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Tocris/Janelia Fluor<sup>®</sup> 646, SE/6148/5/5 mg

Tocris/Janelia Fluor^® 646, SE/6148/5/5 mg

价格: ¥3900.00 市场价: 6500.00

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货号: 6148/5

品牌: Tocris

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Description:Cellpermeablefluorescentdye;suppliedasNHSester

AlternativeNames:JF646,NHS

ChemicalName:1-[7-(1-Azetidinyl)-10-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium,innersalt

BIOLOGicalActivityTechnicalDataSolubilityCalculatorsDatasheetsReferences

BiologicalActivity

Fluorescentdye;suppliedasanNHSesterforcouplingtoprimaryaminegroups.CompatIBLewithself-labelingtagsystems(e.g.HaloTag^®andSNAPtag^®).Suitableforconfocalfluorescentimaging,superresolutionmicroscopy(SRM)techniquessuchasdSTORM(liveandfixedcells)andPAINTimaging.CanbemultiplexedfortwocolourimagingwithJaneliaFluor^®549SE(Cat.No.6147).Cellpermeable.Excitationmaximum=646nm;emissionmaximum=664nm.Quantumyield=0.54,Extinctioncoefficient=152,000M^-1cm^-1;A280correctionfactoris0.19.

HaloTagisatrademarkofPromegaCorporation,andSNAP-tagisatrademarkofNewEnglandBiolabs,Inc.

LicensingInformation

SoldunderlicensefromtheHowardHughesMedicalInstitute,JaneliaResearchCampus

ApplicationofJaneliaFluor^®DyesinCardiacTissue

Application of Janelia Fluor® Dyes in Cardiac Tissue

Super-resolutionimage(dSTORM)ofmuscletissuefromtheleftventricleofanadultpigheart.JF646(Cat.No.6148)wasusedtolabelcollagenVI,whichispresentonthecellplasmamembrane,andimagedwitha642nmexcitationlaser.Aclearerstructureisachievedincomparisonwiththewidefieldimage(topright).Scalebar2μm.

ImagekindlyprovidedbyProf.ChristianSoeller,UniversityofExeter;acquiredbyAlexClowsleyandAnnaMeletiou.

TechnicalData

M.Wt	593.71
Formula	C₃₃H₃₁N₃O₆Si
Storage	Storeat-20°C
CASNumber	1811539-59-9
PubChemID	124201858
InChIKey	ODBCGJUBBYOJOS-UHFFFAOYSA-N
Smiles	O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CCC7)=C2

Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.

AllTocrisproductsareintendedforlaboratoryresearchuseonly.

SolubilityData

	Solvent	MaxConc.mg/mL	MaxConc.mM
Solubility
Solubility	DMSO	59.37	100

PreparingStockSolutions

Thefollowingdataisbasedontheproductmolecularweight593.71.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.

Concentration/SolventVolume/Mass	1mg	5mg	10mg
1mM	1.68mL	8.42mL	16.84mL
5mM	0.34mL	1.68mL	3.37mL
10mM	0.17mL	0.84mL	1.68mL
50mM	0.03mL	0.17mL	0.34mL

ProductDatasheets

CertificateofAnalysis/ProductDatasheet

SafetyDatasheet

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