Properties:
RelatedCategories | 5micronDiscoveryBIOWidePore(300)C5Columns,Analytical/Chromatography,DiscoveryBIO,DiscoveryBIOHPLCColumns,DiscoveryBIOWidePore(300Å)C5, |
feature | endcapped |
extentoflabeling | 3.5%CarbonloADIng |
application(s) | HPLC:suitable |
L×I.D. | 25 cm×4.6 mm |
surfacecoverage | 4.5 μmol/m2 |
matrix | silicaparticleplatform |
matrixactivegroup | C5(pentyl)phase |
particlesize | 5 μm |
poresize | 300 Å |
surfacearea | 100 m2/g |
modeofchromatography | Reversedphase |
Description:
Generaldescription
DiscoveryBIOWidePoreC5wasdesignedfortheefficientandreliableseparationofproteinsandpeptides,especiallyhydrophobicpeptides,byRP-HPLC.Long-chainphases,likeC8orC18,areoftentoohydrophobicforproteinsandcancauseexcessivelylongretentiontimeorevenirreversIBLebindingtothecolumn.Forthisreasonshort-chainphases,typicallyC3orC4,areoftenusedforRP-HPLCofproteins.However,theseshort-chainphasesaresusceptibletohydrolysis,resultinginshortcolumnlifetime,especiallyatlowpH.TheDiscoveryBIOWidePoreC5giveselutionordersimilartoaconventionalC4,yethasenhancedpHstABIlityforlongercolumnlifetime.Generally,higherefficiencyseparationsareachievableontheDiscoveryBIOWidePoreC5becauseoftheimprovementswehavemadetothesilicaandbonded-phasechemistry.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
LegalInformation
DiscoveryisaregisteredtrademarkofSigma-AldrichCo.LLC