Properties:
RelatedCategories | AnalyticalColumn,3μm,Analytical/Chromatography,DiscoveryHPLCColumns,DiscoveryHSF5,HPLC, |
feature | endcapped |
extentoflabeling | 12%CarbonloADIng |
parameter | ≤70°Ctemp.range |
application(s) | HPLC:suitable |
L×I.D. | 10 cm×2.1 mm |
surfacecoverage | 4 μmol/m2 |
impurities | <10 ppmmetals |
matrix | silicagel,highpurity,sphericalparticleplatform |
matrixactivegroup | PFP(pentafluorophenyl)phase |
particlesize | 3 μm |
poresize | 120 Å |
surfacearea | 300 m2/g |
operatingpHrange | 2-8 |
suitABIlity | suitableforL43perUSP |
modeofchromatography | Hydrophilicinteraction(HILIC) |
| Reversedphase |
Description:
FeaturesandBenefits
• Uniqueselectivity
• SimilarretentiontoC18(sometimesrequiresstrongermobilephase)
• Excellentpeakshape
• Stable,low-bleedLC-MSseparations
• Scalableseparationsfrom3to10μmparticlesizes
Generaldescription
GuidelinesfortransferringaC18methodtoDiscovery®HSF5:
Generally,basesareretainedlongerontheHSF5thanonaC18.IncreasingtheorganiccontentofaC18separation5to10percentwillgenerallyprovidesimilarretentiononanHSF5.Resultswithothercompoundsarehighlyvariable.However,itisgenerallytruethatsoluteswithlog Po/wvalueslessthan2.5willberetainedlongeronHSF5comparedtoaC18.Thedegreeofdifferenceishighlysolutedependent.
TheDiscovery®HSF5bondedphaseprovidesreversed-phaseseparationsthataredistinctlydifferentfromC18columns.However,compoundswillgenerallyelutewithinthesameretentiontimewindow,makingmostC18methodseasilytransferable.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
LegalInformation
DiscoveryisaregisteredtrademarkofSigma-AldrichCo.LLC