Boc-protectedCyanine5hydrazidereactivedyeforthelabelingofaldehydesandketones.
Boc-(tert-butoxycarbonyl)protectivegroupcanberemovedwithtrifluoroaceticacid,orotherstrongacid.Reactivehydrazideisformed,whichcanthencouplewithvariouscarbonylgroupsyieldingCyanine5-labeledmolecules.
Generalproperties
Appearance: | darkbluepowder |
Molecularweight: | 633.26 |
Molecularformula: | C37H49ClN4O3 |
Solubility: | goodindichloromethane,polarorganicsolvents(DMF,DMSO),alcohols,lowinwater |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight. |
Spectralproperties
Excitationmaximum,nm: | 646 |
ε,L⋅mol−1⋅cm−1: | 250000 |
Emissionmaximum,nm: | 662 |
Fluorescencequantumyield: | 0.2 |
CF260: | 0.03 |
CF280: | 0.04 |