Properties:
RelatedCategories | Analytical/Chromatography,AscentisExpressColumnsforFastHPLC,AscentisExpressF5,5μmHPLCColumns,F5,5μmColumns,HPLC, |
feature | endcapped |
parameter | 60°Ctemp.range |
application(s) | HPLC:suitable |
L×I.D. | 10 cm×4.6 mm |
impurities | <5 ppmmetals |
matrix | Fused-Coreparticleplatform |
matrixactivegroup | PFP(pentafluorophenyl)phase |
particlesize | 5 μm |
poresize | 90 Åporesize |
operatingpH | 1-9 |
suitABIlity | suitableforL43perUSP |
modeofchromatography | Hydrophilicinteraction(HILIC) |
| Reversedphase |
Description:
FrequentlyAskedQuestions
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Generaldescription
Ascentis®Express5μmF5isahigh-speed,high-performanceliquidchromatographycolumnbasedonthehighlyefficientFused-Core®particledesign.Thebonded,endcapped,dimethylpentafluorophenyl-propylsilanestationaryphaseofAscentisExpress5μmF5providesastable,reversed-phasepackingwithelectron-deficientphenylringsduetothepresenceofelectronegativefluorines.Inadditiontoformingπ-πandmildlystericinteractions,F5phasesalsoretaincompoundsbypolarinteractions.Asaresultofhavingbothpolarandnon-polarcharacter,F5phasescanshowdual-moderetentionbehavior,sometimesproducinga“U-shaped”retentionasafunctionofacetonitrilecontentofthemobilephase,withretentionincreasingatbothlowandhighconcentrationsofACN(reversed-phaseandHILICretentionmodes).AscentisExpress5μmF5canbeusedforbasic,acidic,orneutralcompoundswithalternateselectivityfromC18.Recommendedproducts
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LegalInformation
AscentisisaregisteredtrademarkofSigma-AldrichCo.LLC
Fused-CoreisaregisteredtrademarkofAdvancedMaterialsTechnology,Inc.
Safety:
RIDADR
NONHforallmodesoftransport