Content:1mLOverview
The7-AADStainingSolutionisaready-to-usereagentsuitablefortheexclusionofdeadandapoptoticcellsfromflowcytometricanalysis.
Details
Backgroundinformation
7-AAD(7-amino-actinomycinD)isafluorescentdyethatintercalatesintodouble-strandedDNA(GCrichregions).Itisexcludedfromviablecells,butcanpenetratecellmembranesofdeadordyingcells.Therefore,itcanbeusedinsteadofpropidiumiodide(PI)fortheevaluationofcelldeathandapoptosis.
Thefluorescenceemissionmaximumfor7-AADisat647nm.Whenexcitedat488nm,7-AADisdetectedintheredfluorescencechannelcommonlyusedforR-phycoerythrin(PE)-Cy®5tandemdyedetection,withminimalspectraloverlapintotheyellowfluorescencechannelcommonlyusedforPEdetection.