Description
RoswellParkMemorialInstitute(RPMI)1640mediumwasoriginallydevelopedtoculturehumanleukemiccellsins
USPensionandasamonolayer.RPMI1640hassincebeenfoundsuitableforavarietyofmammaliancellsincludingHeLa,Jurkat,MCF-7,PC12,PBMC,astrocytes,andcarcinomas.Weofferavarietyof
Gibco®RPMI1640modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisRPMI1640ismodifiedasfollows:With | Without |
•PhenolRed | •L-glutamine |
Thecompleteformulationisavailable.
Gibco®RPMI1640isuniquefromothermediabecauseitcontainsthereducingagentglutathioneandhighconcentrationsofvitamins.RPMI1640containsbiotin,vitaminB
12,andPABAwhicharenotfoundinEagle"sMinimalEssentialMediumorDulbecco"sModifiedEagleMedium.Inaddition,thevitaminsinositolandcholinearepresentinveryhighconcentrations.
cGMPManufacturingandQualitySystemGibco®RPMI1640ismanufacturedatacGMPcompliantfacilitylocatedinPaisley,Scotland,UK.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
RPMI1640containsnoproteins,lipids,orgrowthfactors.Therefore,RPMI1640requiressupplementation,commonlywith10%FetalBovineSerum(FBS).RPMI1640usesasodiumbicarbonatebuffersystem(2.0g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
ForInVitroDiagnosticUse.