ApplicationComments | TheantibodydetectedacetylatedhistoneH4inacid-extractedproteinsfromHeLacells.Cross-reactswithacetylatedhistoneH2BfromTetrahymenaandweaklycross-reactswithhistoneH2BfromHeLacells.Maycross-reactwithotheracetylatedproteins.Usingsodiumbutyrate,aninhibitorofhistonedeacetylases,enhancesdetectionofacetylatedhistoneH4.Forimmunocytochemistry,humanA431cellsweretreatedwith5mMsodiumbutyrate,aninhibitorofhistonedeacetylases,for24handfixedwithice-cold1%paraformaldehydeinPBS.Variablesassociatedwithassayconditionswilldictatetheoptimaldilution. |