Huzzah® NBD-So
Huzzah® NBD-So, Human Serum Albumin / NBD Sphingosine Complex
Avanti’s new HSA lipid delivery system, Huzzah™, improves the cellular delivery of NBD-sphingosine through its conjugation with human serum albumin (HSA), a physiologically relevant carrier protein. This conjugate system eliminates the need for organic solvents, which are well-known to adversely affect viability and the phenotypic characteristics of cells.The Huzzah™ lipid delivery system is supplied as a lyophilized conjugate which can be directly and easily reconstituted in water or a buffer of choice and is ideal for studying the effects of poorly soluble bioactive molecules.
Solubility: Reconstitute lyophilized conjugate in 5 mL of water or other aqueous buffer. Exact concentration will vary slightlyfrom batch to batch. Refer to label and/or CoA for exact product concentration upon reconstitution as determined by mass spectrometric analysis. A short sonication may be necessary to ensure complete dissolution. If necessary, filter sterilize the reconstituted conjugate prior to use in cell culture applications.
Molar Ratio: NBD-So:albumin (1:1)Albumin Biological Characterization: microbial free; endotoxin < 1 IU/mLAlbumin Lipid Characterization: ~0.07g/L phospholipids; ~0.02 g/L sphingolipids; ~0.1 g/L fatty acids; and triglycerides and cholesterols below the limit of quantitation of 0.1 g/L and 0.05 g/L
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