Description
ThermoScientific™SuperSignal™WestPicoPLUSChemiluminescentSubstrateisanenhancedchemiluminescent(ECL)horser
ADIshperoxidase(HRP)substratethatenablespicogram-tohighfemtogram-levelproteindetectionbywesternblotanalysis.
SuperSignalWestPicoPLUSsubstrateisdesignedtoprovideexcellentsignalintensityandsensitivityforwesternblottingwithHRPconjugates.Theintensityofthelightemissioncombinedwiththeexceptionaldurationallowsfortheacquisitionofmultipleexposurestomoreeasilyobtainpublication-qualityblotimages.SuperSignalWestPicoPLUSsubstrateiscompat
IBLewithdifferentmembranes,blockingreagents,andawiderangeofantibodydilutionsmakingitanidealchoiceformostwesternblottingapplications.
FeaturesofSuperSignalWestPicoPLUSChemiluminescentSubstrate:
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Picogramtofemtogramsensitivity—detectlow-picogramtohigh-femtogramamountsoftargetproteinonnitrocelluloseorPVDFmembrane
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HighsignalstABIlity—incubatedblotsprovidestablesignaldurationoverthecritical4hourtimeperiodwithupto24hoursoflightoutputunderoptimalconditions
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Stablereagent—8-hourworkingsolutionstability;1-yearkitstabilityatroomtemperature
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Economical—optimizedfordiluteantibodyconcentrations:
--0.2to1.0µg/mLprimaryantibody(1:1000to1:5000dilutionfrom1mg/mLstock)
--10to50ng/mLsecondaryantibody(1:20,000to1:100,000dilutionfrom1mg/mLstock)
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Exceptionalrobustness—provideshighperformanceoutsideoftherecommendedantibodydilutionranges,includingthemostcommon1:5Kto1:10Ksecondaryantibodydilutionsofa1mg/mLstocksolution
ForResearchUseOnly.Notforuseindiagnosticprocedures.