Description
MinimumEssentialMedium(MEM)αiswidelyusedformammaliancellcultureaswellasselectionfortransfectedDHFRnegativecells.MEMαcanbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingkeratinocytes,primaryratastrocytes,andhumanmelanomacells.Weofferavarietyof
Gibco®MEMαmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisMEMαismodifiedasfollows:
With |
•Ribonucleosides |
•Deoxyribonucleosides |
•PhenolRed |
Thecompleteformulationisavailable.
Gibco®MEMαisamodificationofMinimumEssentialMedium(MEM)thatcontainsnon-essentialaminoacids,sodiumpyruvate,lipoicacid,vitaminB
12,biotin,andascorbicacid.MEMαisavailablewithoutnucleosidesforuseasaselectionmediumforDG44andotherDHFRnegativecells.Gibco®MEMαwithGlutaMAX™supplementminimizestoxicammoniabuild-upandimprovescellvi
ABIlityandgrowthinaneasy-to-useformat.ThisproductismadewithEarle’ssalts.
ProductIntendedUseFor
invitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.
cGMPManufacturingandQualitySystemGibco®MEMαismanufacturedatacGMPcompliantfacility,locatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferanidenticalGibco®MEMαproductmadeinourScotlandfacility(32571-028).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
MEMαcontainsnoproteins,lipids,orgrowthfactors.Therefore,MEMαrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).MEMαusesasodiumbicarbonatebuffersystem(2.2g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
ForInVitroDiagnosticUse.