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Millipore/2750 | BrdU Cell Proliferation Kit/2750/200 assays
  • Millipore/2750 | BrdU Cell Proliferation Kit/2750/200 assays

Millipore/2750 | BrdU Cell Proliferation Kit/2750/200 assays

价格: ¥4068.00 市场价: 6780.00

货号: 2750
品牌: Millipore
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    • Description
      CatalogueNumber2750
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionBrdUCellProliferationKit
      OverviewTestPrinciple

      WithCHEMICON®"sBrdUCellProliferationAssayKit,mousemonoclonalantibodyisusedtodetecttheBrdUinasample.Afteradditionofgoatanti-mouseIgG-peroxidaseconjugatedsecondaryantibody,substrateandstopsolution,theamountofBrdUisdetermined.ThehighertheOD,thehighertheBrdUconcentrationinthesample.
      MaterialsRequiredbutNotDelivered1.Pipettors&tipscapableofaccuratelymeasuring2-1000μL

      2.Multi-channelorrepeatingPipettes

      3.Washbottleormultichanneldispensorforwashing

      4.2000mLgraduatedcylinder

      5.PBS(137mMNaCl,2.7mMKCl,4.3mMNa2HPO4-7H2O,1.4mMKH2PO4)

      6.DeionizedordistilledH2O.

      7.96-wellmicroplatereaderwith450-540nmor450-595nmdualwavelengthor450nmsinglewavelengthfilter

      8.Tissueculturemicroplate(96wellculturedish)

      9.Sterilereagenttroughs

      10.Microsyringefilter(0.2μm)

      11.Syringe
      BackgroundInformationEvaluationofcellcycleprogressionisessentialforinvestigationsinmanyscientificfields.Measurementof[3H]thymidineincorporationascellsenterSphasehasbeenatrADItionalmethodfordetectionofcellproliferation.Subsequentquantificationof[3H]thymidineisperformedbyscintillationcountingofautoradiography.Thistechnologyisslow,laborintensiveandhasseverallimitationsincludingthehandlinganddisposalofradioisotopesandthenecessityofexpensiveequipment.

      Awellestablishedalternativeto[3H]thymidineuptakehasbeendemonstratedbynumerousresearchers.Inthesemethodsbromodeoxyuridine(BrdU),athymidineanalog,replaces[3H]thymidine.BrdUisincorporatedintonewlysynthesizedDNAstrandsofactivelyproliferatingcells.FollowingpartialdenaturationofdoublestrandedDNA,BrdUisdetectedimmunochemicallyallowingtheassessmentofthepopulationofcellswhicharesynthesizingDNA.
      ProductInformation
      Components
      • BrdUReagent:(PartNo.2750a)One15μLvialofa500XsolutionofBrdU.
      • FixingSolution:(PartNo.2750b)One40mLbottle.
      • PredilutedBrdUDetectionAntibody:(PartNo.2750c)One20mLvialofananti-BrdUsolution.
      • StopSolution:(PartNo.2750d):One25mLbottleof2.5Nsulfuricacidsolution.
      • Goatanti-MouseIgG,Peroxidaselabeled:(PartNo.2750e)One15μLvialofa2000Xsolution.
      • ConjugateDiluent:(PartNo.2750f)One25mLbottle.
      • Substrate:(PartNo.2750g)One25mLbottle,readytouseTMB.
      • 50XPlateWashConcentrate:(PartNo.2750h)One90mLbottleofbufferedTriswithsurfactant.
      DetectionmethodColorimetric
      StorageandShippingInformation
      StorageConditionsMaintainthekitat2°to8°Cundersterileconditionsforupto6monthsafterdateofreceipt.

      Beforefirstuse:removetheFixativeSolutionandplaceatroomtemperatureforatleast4hourspriortouse.Precipitatesthatmayoccurduringcoldstorageshouldgobackintosolution.
      Applications
      ApplicationTheBrdUCellProliferationAssayKitisusedtodetecttheBrdUinasample.
      KeyApplications
      ApplicationNotesTheCHEMICON®BrdUCellProliferationAssayKitisanon-isotopicassayfortheinvitroquantitativedetectionofnewlysynthesizedDNAofactivelyproliferatingcells.

      ForResearchUseOnly.Notforuseindiagnosticprocedures.
      BIOLOGicalInformation
      SpeciesReactivity
      • Mouse
      • Human
      • Rat
      • Pig
      • Horse
      • Rabbit
      • GuineaPig
      • Hamster
      • Non-humanPrimate
      • Canine
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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