Description
RoswellParkMemorialInstitute(RPMI)1640mediumwasoriginallydevelopedtoculturehumanleukemiccellsins
USPensionandasamonolayer.RPMI1640hassincebeenfoundsuitableforavarietyofmammaliancellsincludingHeLa,Jurkat,MCF-7,PC12,PBMC,astrocytesandcarcinomas.Weofferavarietyof
Gibco®RPMI1640modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisRPMIismodifiedasfollows:With | Without |
•L-glutamine | •HEPES |
Thecompleteformulationisavailable.
Gibco®RPMI1640isuniquefromothermediabecauseitcontainsthereducingagentglutathioneandhighconcentrationsofvitamins.RPMI1640containsbiotin,vitaminB
12andPABAwhicharenotfoundinEagle"sMinimalEssentialMediumorDulbecco"sModifiedEagleMedium.Inaddition,thevitaminsinositolandcholinearepresentinveryhighconcentrations.
ProductUseForResearchUseOnly:Notintendedforanimalorhumandiagnosticortherapeuticuse.
cGMPManufacturingandQualitySystemGibco®RPMI1640ismanufacturedatacGMPcompliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
RPMI1640containsnoproteins,lipids,orgrowthfactors.Therefore,RPMI1640requiressupplementation,commonlywith10%FetalBovineSerum(FBS).RPMI1640usesasodiumbicarbonatebuffersystem(2.0g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
ForResearchUseOnly.Notforuseindiagnosticprocedures.