Description
ThermoScientificPageRulerPlusPrestainedProteinLadderisamixtureofnine(9)blue-,orange-andgreen-stainedproteins(10to250kDa)foruseassizestandardsinproteinelectrophoresis(SDS-PAGE)andWesternblotting.
FeaturesofPageRulerPlusPrestainedProteinLadder:
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Sizerange—nineproteinsspanning10to250kDa
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Ready-to-use—suppliedinalo
ADIngbufferfordirectloadingongels;noneedtoboil
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Sharpbands—color-codedbandsofsimilarintesityforeasyvisualization
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Qualitytested—eachlotevaluatedbySDS-PAGEandWesternblotting
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Brightreferencebands—orangeat70and25kDa,andgreenat10kDa
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Membrane-compatIBLe—coloredbandstransfertomembranesforWesternblotting
ThisprestainedproteinMW
MarkerisdesignedformonitoringtheprogressofSDS-polyacrylamidegelelectrophoresis,forassessingtransferefficiencyontoPVDF,nylonandnitrocellulosemembranes,andforestimatingtheapproximatesizeofseparatedproteinsthathavebeenmadevisiblewithgelstainsorWesternblotdetectionreagents.Abluechromophoreisboundtoallproteins,exceptproteinsoftworeferencebandsof70kDaand25kDathatarecoloredwithanorangedyeandonegreenreferencebandof10kDa.PageRulerPlusPrestainedProteinLadderisreadytouse:noheating,furtherdilutionoradditionofareducingagentisrequiredbeforeloadingontoagel.
Includes:•Dye-stainedproteinsin62.5mMTris-H3PO4(pH7.5at25°C),1mMEDTA,2%SDS,10mMDTT,1mMNaN3and33%glycerol.
Applications:•MonitoringproteinmigrationduringSDS-polyacrylamidegelelectrophoresis
•MonitoringproteintransferontomembranesafterWesternblotting
•SizingofproteinsonSDS-polyacrylamidegelsandWesternblots
ForResearchUseOnly.Notforuseindiagnosticprocedures.