Fluorescein(FAM)isabrightfluorophorethathasfoundanextremelywideapplicationinlifescienceresearch.Thefluorophoreishydrophilicanditsderivativespossessgoodsolubilityinaqueousbuffers.
FluoresceiniscompatIBLewithawidevarietyoffluorescentinstrumentation-mostinstrumentsareequippedwithFAMchannelfilters.
Thisproductcontainsthepure6-isomeroffluorescein.
Recommendedprotocol
NHSesterlabelingofaminobiomolecules
Generalproperties
Appearance: | yellowcrystallinesolid |
MassspecM+increment: | 358.0 |
Molecularweight: | 473.39 |
Molecularformula: | C25NO9H15 |
IUPACname: | (2,5-dioxopyrrolidin-1-yl)3",6"-dihydroxy-1-oxospiro[2-benzofuran-3,9"-xanthene]-5-carboxylate |
Solubility: | goodinDMF,DMSO |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
Excitationmaximum,nm: | 494 |
ε,L⋅mol−1⋅cm−1: | 75000 |
Emissionmaximum,nm: | 520 |
Fluorescencequantumyield: | 0.9 |
CF260: | 0.20 |
CF280: | 0.17 |