Description
The
ThermoScientific™
Pierce™QuantitativeColorimetricPeptideAssayisaneasy-to-usecolorimetricmicroplateassaydesignedspecificallytoimprovethesensitivityandreproducibilityofquantitationofpeptidemixtures.
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Sensitive—accuratelydetectaslittleas25µg/mLofpeptidemixture
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Robust—assayperformancerigorouslytestedusingpeptidedigestmixtures
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Robustpeptidedigeststandard—kitincludesavalidatedpeptidedigeststandardforimprovedreproducibilityofquantitation
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CompatIBLe—workswithmanyreagents,includingmassspectrometrysamplepreparationreagentsandTMT™-labeledpeptides
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Convenient—easymix-andread-formatandstablecolorimetricsignalthatmaybereadinaslittleas15minutes
ThePierceQuantitativeColorimetricPeptideAssayprovidesmodifiedBCAreagentsforthereductionofCu+2toCu+1andaproprietarychelatorthathavebeenoptimizedforthequantitationofpeptidemixtures.Inthisreaction,thecopperisfirstreducedbytheamidebackboneofpeptidesunderalkalineconditions(Biuretreaction).Theproprietarychelatorthencoupleswiththereducedcoppertoformabrightredcomplexwithabsorbanceof480nm.Thesignalproducedfromthisreactionis3-4foldmoresensitivethantheMicro-BCAProteinAssayforpeptideanalysis.
Thekitalsocontainsahighqualitypeptidedigestreferencestandardforuseingeneratinglinearstandardcurvesandtoserveasacalibrationcontrol.Thiscolorimetricpeptideassayrequiresasmallamountofsample(20µL)andhasaworkingpeptideconcentrationrangeof25–1000μg/mL.Theincreasedsensitivity,lowsampleassayvolume,andincludedreferencestandardareessentialforaccurateandrobustmeasurementofpeptidedigestsamples,especiallyformassspectrometryapplications.
Applications:•Recommendedforpeptidemixtures
•Notrecommendedforsyntheticpeptidesbecausetheassayisaffectedbypeptideaminoacidcontent
•Normalizingsampleconcentrationsforquantitativedownstreamapplications
•Normalizingsamplelo
ADIngvolumesforLC,MS,andLC/MSapplications
•Measuringrecoveryafterpeptideclean-upprocedures
ForResearchUseOnly.Notforuseindiagnosticprocedures.