Description
Opti-MEM®Reduced-SerumMediumisanimprovedMinimalEssentialMedium(MEM)thatallowsforareductionofFetalBovineSerumsupplementationbyatleast50%withnochangetogrowthrateormorphology.Opti-MEM®isalsorecommendedforusewithcationiclipidtransfectionreagents,suchasLipofectamine™reagent.Opti-MEM®canbeusedwithavarietyofs
USPensionandadherentmammaliancells,includingSp2,AE-1,CHO,BHK-21,HEK,andprimaryfibroblasts.Weofferavarietyof
Gibco®Opti-MEM®modificationsforarangeofcellcultureapplications.
ThisOpti-MEM®ismodifiedasfollows:
Thecompleteformulationisconfidential.Formoreinformation,pleasecontactTechnicalServices.
Gibco®Opti-MEM®isauniquemediathatcontainsinsulin,transferrin,hypoxanthine,thymidine,andtraceelements.Theseadditionalcomponentsallowforareductioninserumsupplementationbyatleast50%.
ProductIntendedUseFor
invitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.
cGMPManufacturingandQualitySystemForsupplychaincontinuity,wemanufactureGibco®Opti-MEM®attwoseparatefacilitieslocatedinGrandIsland,NYandScotland,UK.BothsitesarecompliantwithcGMPmanufacturingrequirements,arecertifiedtoISO13485,andareregisteredwiththeFDAasmedicaldevicemanufacturers.
Gibco®Opti-MEM®usesasodiumbicarbonatebuffersystem(2.4g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
PowderformsofGibco®cellculturemediumrequiresodiumbicarbonatesupplementation,pHadjustmentandfiltrationatthetimeofpreparation(seeprotocolfordetails).