Description
RoswellParkMemorialInstitute(RPMI)1640mediumwasoriginallydevelopedtoculturehumanleukemiccellsins
USPensionandasamonolayer.RPMI1640hassincebeenfoundsuitableforavarietyofmammaliancellsincludingHeLa,Jurkat,MCF-7,PC12,PBMC,astrocytes,andcarcinomas.Weofferavarietyof
Gibco®RPMI1640modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisRPMIismodifiedasfollows:
With | Without |
PhenolRed | L-glutamine |
HEPES | |
Thecompleteformulationisavailable.
Gibco®RPMI1640isuniquefromothermediabecauseitcontainsthereducingagentglutathioneandhighconcentrationsofvitamins.RPMI1640containsbiotin,vitaminB
12andPABAwhicharenotfoundinEagle"sMinimalEssentialMediumorDulbecco"sModifiedEagleMedium.Inaddition,thevitaminsinositolandcholinearepresentinveryhighconcentrations.ThisRPMIformulationhastheDutchmodification(additionofHEPES,withsodiumbicarbonateloweredto1g/L).
ProductIntendedUseForResearchUseOnly.Notforuseindiagnosticprocedures.
cGMPManufacturingandQualitySystemGibco®RPMI1640ismanufacturedatacGMPcompliantfacility,locatedinPaisley,Scotland,UK.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
RPMI1640containsnoproteins,lipids,orgrowthfactors.Therefore,RPMI1640requiressupplementation,commonlywith10%FetalBovineSerum(FBS).RPMI1640usesasodiumbicarbonatebuffersystem(1.0g/L)andthereforerequiresa5-7%CO
2environmenttomaintainphysiologicalpH.
ForResearchUseOnly.Notforuseindiagnosticprocedures.