Description
Dulbecco"sModifiedEagleMedium(DMEM)isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEMincludeprimaryfibroblasts,neurons,glialcells,HUVECs,andsmoothmusclecells,aswellascelllinessuchasHeLa,293,Cos-7,andPC-12.Weofferavarietyof
Gibco®DMEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisDMEMismodifiedasfollows:
With | Without |
•HighGlucose | •L-glutamine |
•PhenolRed | •SodiumPyruvate |
| •HEPES |
Thecompleteformulationisavailable.
DMEMisuniquefromothermediaasitcontains4timestheconcentrationofaminoacidsandvitaminsthantheoriginalEagle"sMinimalEssentialMedium.DMEMwasoriginallyformulatedwithlowglucose(1g/L)andsodiumpyruvate,butisoftenusedwithhigherglucoselevels,withorwithoutsodiumpyruvate.
ProductUseForResearchUseOnly:Notintendedforanimalorhumandiagnosticortherapeuticuse.
cGMPManufacturingandQualitySystemGibco®DMEMismanufacturedatacGMPcompliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
DMEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,DMEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).DMEMusesasodiumbicarbonatebuffersystem(3.7g/L)andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.