Description
DMEM/F-12(Dulbecco"sModifiedEagleMedium/NutrientMixtureF-12)isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEM/F-12includeMDCK,glialcells,fibroblasts,humanendothelialcells,andratfibroblasts.WeofferavarietyofDMEM/F-12modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.
ThisDMEM/F-12ismodifiedasfollows:With | Without |
•L-glutamine | •HEPES |
Thecompleteformulationisavailable.
UsingDMEM/F-12DMEM/F-12isa1:1mixtureofDMEMandHam"sF-12.ThisformulationcombinesDMEM"shighconcentrationsofglucose,aminoacidsandvitaminswithF-12"swidevarietyofcomponents.DMEM/F-12containsnoproteins,lipids,orgrowthfactors.Therefore,DMEM/F-12mayrequiresupplementation,commonlywith10%FetalBovineSerum(FBS).DMEM/F-12usesasodiumbicarbonatebuffersystemandthereforerequiresa5–10%CO
2environmenttomaintainphysiologicalpH.
cGMPmanufacturingandqualitysystemForsupplychaincontinuity,wemanufactureDMEM/F-12attwoseparatefacilities,locatedinGrandIsland,NYandScotland,UK.BothsitesarecompliantwithcGMP-manufacturingrequirements,arecertifiedtoISO13485,andareregisteredwiththeFDAasmedicaldevicemanufacturers.
ForResearchUseOnly.Notforuseindiagnosticprocedures.