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Millipore/17-671 | ChIPAb+ Trimethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-6
  • Millipore/17-671 | ChIPAb+ Trimethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-6

Millipore/17-671 | ChIPAb+ Trimethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-6

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货号: 17-671
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    • Description
      CatalogueNumber17-671
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+Trimethyl-HistoneH4(Lys20)-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+Trimethyl-HistoneH4(Lys20)setincludestheAnti-trimethyl-HistoneH4(Lys20)antibody,anegativecontrolsupernatant,andqPCRprimerswhichamplifya110bpregionwithinthepromoterofthehumanβ-globingene.ThetrimethylhistoneH4(Lys20)andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationoftrimethyl-histoneH4(Lys20)associatedchromatin.
      AlternateNames
      • H4K20me3
      • HistoneH4(trimethylK20)
      • H4histonefamily,memberA
      • histone1
      • H4a
      • histonecluster1
      • H4a
      ProductInformation
      FormatCultureSupernatant
      Control
      • Includesnegativecontrolrabbitantibodyandcontrolprimersspecificforhumanβ-globinpromoter.
      PresentationAnti-trimethyl-HistoneH4(Lys20)(rabbitmonoclonalIgG).Onevialcontaining100μLofcellculturesupernatantin0.1%sodiumazide.Storeat-20°C.

      NegativeControlSupernatant.Onevialcontaining100μLcellrabbitmonoclonalculturesupernatantcontaining0.1%sodiumazide.Storeat-20°C.

      ChIPPrimersB-Globin.Onevialcontaining75μLof5μMofeachprimerspecificforhumanβ-globin.Storeat-20°C.
      FOR:AGGACAGGTACGGCTGTCATC
      REV:TTTATGCCCAGCCCTGGCTC
      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
      Applications
      ApplicationThisChIPAb+Trimethyl-HistoneH4(Lys20)-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • WesternBlotting
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromHeLacells(2X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing10μLofeitheranegativecontrolsupernatantorAnti-Trimethyl-HistoneH4(Lys20)antibodyandtheMagnaChIPAKit(PartNo.17-610).SuccessfulimmunoprecipitationoftrimethylhistoneH4(Lys20)associatedDNAfragmentswasverifiedbyqPCRusingβ-globinChIPPrimersversusControlPrimerscorrespondingtotheGAPDHpromoter(Pleaseseefigures).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatin,withimmunoprecipitatedDNAfromnegativecontrolsupernatantshownas(-)andtrimethylhistoneH4(Lys20)shownas(+).
      PleaserefertotheEZ-MagnaChIP™A(Cat.#17-408)orEZ-ChIP™(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      AcidextractsfromHeLacellsuntreatedorpre-absorbedwith1μMoftrimethylated(Lys20)HistoneH4peptide(Lanes1and2respectively)wereprobedwithanti-trimethyl-HistoneH4(Lys20)(1:1,000)(Pleaseseefigures).

      DotBlotAnalysis:AbsuranceHistoneH3AntibodySpecificityArray(Cat.No.16-667)andAbsuranceHistoneH2A,H2B,H4AntibodySpecificityArray(Cat.No.16-665),whichcontainhistonepeptideswithvariousmodificationswereprobedwithCat.No04-079,Anti-trimethyl-HistoneH4(Lys20);dilution1:500.ProteinswerevisualizedusingaDonkeyanti-rabbitIgGconjugatedtoHRPandachemiluminescencedetectionsystem.
      BiologicalInformation
      ImmunogenThetrimethylhistoneH4(Lys20)antibodyismadeagainstasyntheticpeptidecorrespondingtoaminoacids18-22ofhistoneH4.trimethylatedonlysine20.
      Epitopea.a.18-22
      HostRabbit
      SpecificityRecognizeshistoneH4,Mr11kDa
      IsotypeIgG
      SpeciesReactivity
      • Human
      • GreenPlants
      • Vertebrates
      SpeciesReactivityNoteHuman.Nottestedinotherspecies.Theimmunogensequenceisidenticalinawiderangeofanimalandplantspecies,sobroadcross-reactivityisexpected.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6.
      GeneSymbol
      • H4/A
      • H4/B
      • H4/C
      • H4/D
      • H4/E
      • H4/G
      • H4/H
      • H4/I
      • H4/J
      • H4/J
      • H4/M
      • H4/N
      • H4/O
      • H4/a
      • H4F2
      • H4FA
      • H4FB
      • H4FC
      • H4FD
      • H4FE
      • H4FG
      • H4FH
      • H4FI
      • H4FJ
      • H4FK
      • H4FM
      • H4FN
      • H4FO
      • HIST2H4
      Modifications
      • Methylation
      UniProtNumber
      UniProtSummaryFUNCTION:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.

      SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.

      SUBCELLULARLOCATION:Nucleus.

      PTM:AcetylationatLys-6,Lys-9,Lys-13andLys-17occursincodingregionsofthegenomebutnotinheterochromatin.

      CitrullinationatArg-4byPADI4impairsmethylation.

      MonomethylationatArg-4byPRMT1favorsacetylationatLys-9andLys-13.DemethylationisperformedbyJMJD6.

      Monomethylated,dimethylatedortrimethylatedatLys-21.MonomethylationisperformedbySET8.TrimethylationisperformedbySUV420H1andSUV420H2andinducesgenesilencing.

      UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirradiation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.

      Sumoylated,whichisassociatedwithtranscriptionalrepression.

      SEQUENCESIMILARITY:BelongstothehistoneH4family.

      SEQUENCECAUTION:ThesequenceAAI28106.1differsfromthatshown.Reason:Frameshiftatposition3.

      MolecularWeight~11kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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